Paraoxonase 1 Activity Kit
Product name: Paraoxonase Activity Assay Kit 1
Detection method: Fluorescent
Example type: Serum, Plasma
Product description
The Paraoxonase 1 Activity Assay Kit (ab241044) allows rapid measurement of paraoxonase 1 (PON1) activity, using a fluorogenic substrate that is converted to a highly fluorescent product (Ex / Em = 368/460 nm). This ensures dramatically higher sensitivity than UV or colorimetric assays and eliminates the need for hazardous toxic substrates. A selective PON1 inhibitor is provided to verify specific PON1 activity. The assay is simple to perform, adaptable, high throughput, and can detect a minimum of 2.0 µU paraoxonase activity with a 5 µL sample volume.
Platform: Microplate reader
Properties
Storage instructions
Store at -20 ° C. See protocols.
Function
Hydrolyzes the toxic metabolites of a variety of organophosphate insecticides. Capable of hydrolyzing a broad spectrum of organophosphate and lactone substrates, and various aromatic carboxylic acid esters. There is enzymatic protection of low-density lipoproteins against oxidative modification and the consequent series of events that lead to the formation of atheromas.
Tissue specificity
Plasma, associated with HDL (at the protein level). Expressed in the liver, but not in the heart, brain, placenta, lung, skeletal muscle, kidney, or pancreas.
Participation in the disease
Genetic variation in PON1 is associated with susceptibility to microvascular complications of type 5 diabetes (MVCD5) [MIM: 612633]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the leading cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note = Homozygosity of the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients.
Sequence similarities
It belongs to the paraoxonase family.
Post-translational modifications
- Glycosylated.
- The signal sequence is not cleaved.
- Present in two forms, Form B contains a disulfide bond, Form A does not.
Cell location
Secreted> extracellular space.
